abstract：:The sequence of gene xynB encoding xylanase B from Paenibacillus sp. BP-23 was determined. It revealed an open reading frame of 999 nucleotides encoding a protein of 38,561 Da. The deduced amino acid sequence of xylanase B shows that the N-terminal region of the enzyme lacks the features of a signal peptide. When the ...

journal_title：Applied microbiology and biotechnology

authors： Gallardo O,Diaz P,Pastor FI

更新日期：2003-05-01 00:00:00

abstract：:C (2) to C (4) chloroalkanes have been used for a wide range of industrial applications. Consequently, numerous leaks to the environment have occurred. It is generally observed that the lower chlorinated members of the group, containing 1-3 chlorine atoms, accumulate in environments where reductive conditions prevail....

journal_title：Applied microbiology and biotechnology

authors： De Wildeman S,Verstraete W

更新日期：2003-04-01 00:00:00

abstract：:The genus Aspergillus is widely used for the production of plant cell wall polysaccharide-degrading enzymes. The range of enzymes purified from these fungi covers nearly every function required for the complete degradation of cellulose, xyloglucan, xylan, galacto(gluco)mannan and pectin. This paper describes the Asper...

journal_title：Applied microbiology and biotechnology

authors： de Vries RP

更新日期：2003-03-01 00:00:00

abstract：:While diatoms are widely present in terms of diversity and abundance in nature, few species are currently used for biotechnologically applications. Most studies have focussed on intracellularly synthesised eicosapentaenoic acid (EPA), a polyunsaturated fatty acid (PUFA) used for pharmaceutical applications. Applicatio...

journal_title：Applied microbiology and biotechnology

authors： Lebeau T,Robert JM

更新日期：2003-02-01 00:00:00

abstract：:Substrate oxidation of aromatic substances by the enzyme laccase followed by a heteromolecular coupling with a co-substrate is a promising possibility for the synthesis of new compounds. To find a suitable reactor for the effective production of new compounds, the laccase-catalysed coupling of 3-(3,4-dihydroxyphenyl)p...

journal_title：Applied microbiology and biotechnology

authors： Pilz R,Hammer E,Schauer F,Kragl U

更新日期：2003-02-01 00:00:00

abstract：:The genus Trichoderma comprises a group of filamentous ascomycetes that are now widely used in industrial applications because of their ability to produce extracellular hydrolases in large amounts. In addition, strong inducible promoters together with high secretory capacity have made Trichoderma an attractive host fo...

journal_title：Applied microbiology and biotechnology

authors： Mach RL,Zeilinger S

更新日期：2003-01-01 00:00:00

abstract：:Palm-oil mill fiber (POMF) is a fibrous, natural hard material discharged in enormous amounts from palm-oil mills in tropical plantations; therefore, research to find microorganisms that decompose POMF was conducted. As the result of screening, a new thermophilic fungus, Chaetomium sp. nov. MS-017, exhibiting rapid gr...

journal_title：Applied microbiology and biotechnology

authors： Suyanto,Ohtsuki T,Yazaki S,Ui S,Mimura A

更新日期：2003-01-01 00:00:00

abstract：:Metabolic stress is a phenomenon often discussed in conjunction with recombinant protein production in Escherichia coli. This investigation shows how heterologous protein production and the presence of host cell proteases is related to: (1) Isopropyl-beta- D-thiogalactopyranoside (IPTG) induction, (2) cell-mass concen...

journal_title：Applied microbiology and biotechnology

authors： Ramchuran SO,Nordberg Karlsson E,Velut S,De Maré L,Hagander P,Holst O

更新日期：2002-12-01 00:00:00

abstract：:Pseudomonas rhodesiae KK1 was isolated from a former manufactured-gas plant site, due to its ability to grow rapidly in a mixture of polycyclic aromatic hydrocarbons (PAHs). Radiorespirometric analysis revealed that strain KK1 was found to be able to mineralize anthracene, naphthalene and phenanthrene. Notably, phenan...

journal_title：Applied microbiology and biotechnology

authors： Kahng HY,Nam K,Kukor JJ,Yoon BJ,Lee DH,Oh DC,Kam SK,Oh KH

更新日期：2002-12-01 00:00:00

abstract：:The main objective of this work was the optimization of the production of the beta-ketolase, acetopyruvate hydrolase, from Pseudomonas putida O1. Orcinol was used as an inducer for enzyme production. The growth medium was optimized in two steps. In the first step, screening for optimal glucose concentration was perfor...

journal_title：Applied microbiology and biotechnology

authors： Hofer H,Mandl T,Steiner W

更新日期：2002-11-01 00:00:00

abstract：:The kinetics of biodegradation of polycyclic aromatic hydrocarbons (PAHs) by a mixed culture were determined in a creosote-contaminated soil and in a pristine soil. A competitive-inhibition model was able to represent the kinetics of degradation of PAHs from the creosote-contaminated soil, from the lag phase through t...

journal_title：Applied microbiology and biotechnology

authors： Lotfabad SK,Gray MR

更新日期：2002-11-01 00:00:00

abstract：:Nitrile-converting enzymes are becoming commonplace in the synthesis of pharmaceuticals and commodity chemicals. These versatile biocatalysts have potential applications in different fields including synthetic biocatalysis and bioremediation. This review attempts to describe in detail the three major classes of nitril...

journal_title：Applied microbiology and biotechnology

authors： Banerjee A,Sharma R,Banerjee UC

更新日期：2002-10-01 00:00:00

abstract：:Mediated electrochemical detection of catabolism in prokaryotic cells is well documented; however, the application of this technique to eukaryotic cells has received less attention. Two catabolic substrate-dependent mediated electrochemical signals were detected in the yeast Saccharomyces cerevisiae. The signal using ...

journal_title：Applied microbiology and biotechnology

authors： Baronian KH,Downard AJ,Lowen RK,Pasco N

更新日期：2002-10-01 00:00:00

abstract：:Aflatoxins are toxic and carcinogenic metabolites of several Aspergillus species. The effect of nitrate on aflatoxin production and expression of the key regulatory genes involved in aflatoxin biosynthesis, aflR and aflJ, were compared among isolates of the S(B) and S(BG) strains of A. flavus. Aflatoxin production by ...

journal_title：Applied microbiology and biotechnology

authors： Ehrlich KC,Cotty PJ

更新日期：2002-10-01 00:00:00

abstract：:The dibenzothiophene (DBT) desulfurizing bacterium metabolizes DBT to form 2-hydroxybiphenyl without breaking the carbon skeleton. Of the DBT desulfurization enzymes, DszC and DszA catalyze monooxygenation reactions, both requiring flavin reductase. We searched for non-DBT-desulfurizing microorganisms producing a flav...

journal_title：Applied microbiology and biotechnology

authors： Ohshiro T,Aoi Y,Torii K,Izumi Y

更新日期：2002-09-01 00:00:00

abstract：:Significant decreases in the apparent viscosity of a bacterial biofilm suspension were measured following addition of sodium, potassium, magnesium, or calcium salts, whereas iron salts increased the viscosity. Electrostatic interactions contribute to biofilm cohesion and iron cations are potent crosslinkers of the bio...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Stewart PS

更新日期：2002-09-01 00:00:00

abstract：:Two recombinant Aeropyrum pernix glutamate dehydrogenase (GDH) enzymes with different length N-termini were cloned and expressed in Escherichia coli: sGDH begins with the amino acid sequence of the extracted native enzyme (M-Q-P-T-D-P-L-E-E), whereas lGDH begins with the sequence of the predicted ORF (M-E-V-L-A-L-Q-P-...

journal_title：Applied microbiology and biotechnology

authors： Helianti I,Morita Y,Murakami Y,Yokoyama K,Tamiya E

更新日期：2002-08-01 00:00:00

abstract：:Vitreoscilla hemoglobin (VHb) has been successfully used to enhance production of foreign proteins in several microorganisms including Escherichia coli. We compared the expression of an oxygen-dependent foreign protein, green fluorescent protein (GFP) under co-expression of VHb in two typical industrial E. coli strain...

journal_title：Applied microbiology and biotechnology

authors： Kang DG,Kim YK,Cha HJ

更新日期：2002-08-01 00:00:00

abstract：:Population dynamics was studied in a 52-l biotrickling filter (BTF) operated for 182 days and used to clean air contaminated with styrene vapors. In the BTF, biomass grew either as free-floating (planktonic) or attached (sessile) microorganisms. PCR-amplified 16S rDNA fragments from planktonic and sessile cells within...

journal_title：Applied microbiology and biotechnology

authors： Tresse O,Lorrain MJ,Rho D

更新日期：2002-08-01 00:00:00

abstract：:In oyster ponds, the marine diatom Haslea ostrearia synthesises and excretes a hydrosoluble pigment of commercial interest called marennin. During the benthic stage, when algal cells are naturally immobilised in their own polysaccharides, marennin production is higher. To optimise this production, axenic cultures of H...

journal_title：Applied microbiology and biotechnology

authors： Lebeau T,Gaudin P,Moan R,Robert JM

更新日期：2002-07-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae has been modified successfully for production of numerous metabolites and therapeutic proteins through metabolic engineering, but has not been utilized to date for the production of lipid-derived compounds. We developed a lipid metabolic engineering strategy in S. cerevisiae based up...

journal_title：Applied microbiology and biotechnology

authors： Dyer JM,Chapital DC,Kuan JW,Mullen RT,Pepperman AB

更新日期：2002-07-01 00:00:00

abstract：:The impact of glucose on glycerol metabolism, especially on 3-hydroxypropionaldehyde (3-HPA) accumulation by resting cells of Lactobacillus reuteri has been investigated. Two systems were used in the study: MRS(-) (modified MRS - omitting glucose, acetate and Tween 80) and distilled water (H(2)O). In MRS(-), addition ...

journal_title：Applied microbiology and biotechnology

authors： Lüthi-Peng Q,Dileme FB,Puhan Z

更新日期：2002-07-01 00:00:00

abstract：:Wastewater produced by the debittering process of green olives (GOW) is rich in polyphenolics and presents high chemical oxygen demand and alkalinity values. Eight white-rot fungi ( Abortiporus biennis, Dichomitus squalens, Inonotus hispidus, Irpex lacteus, Lentinus tigrinus, Panellus stipticus, Pleurotus ostreatus an...

journal_title：Applied microbiology and biotechnology

authors： Aggelis G,Ehaliotis C,Nerud F,Stoychev I,Lyberatos G,Zervakis GI

更新日期：2002-07-01 00:00:00

abstract：:Porcine parvovirus (PPV) virus-like particles (VLPs) constitute a potential vaccine for prevention of parvovirus-induced reproductive failure in gilts. Here we report the development of a large scale (25 l) production process for PPV-VLPs with baculovirus-infected insect cells. A low multiplicity of infection (MOI) st...

journal_title：Applied microbiology and biotechnology

authors： Maranga L,Rueda P,Antonis AF,Vela C,Langeveld JP,Casal JI,Carrondo MJ

更新日期：2002-06-01 00:00:00

abstract：:Two lab-scale plug flow activated sludge reactors were run in parallel for 4 months at 30 and 55 degrees C. Research focussed on: (1) COD (chemical oxygen demand) removal, (2) effluent turbidity at both temperatures, (3) the origin of effluent colloidal material and (4) the possible role of protozoa on turbidity level...

journal_title：Applied microbiology and biotechnology

authors： Vogelaar JC,van Lier JB,Klapwijk B,de Vries MC,Lettinga G

更新日期：2002-06-01 00:00:00

abstract：:Transformants of the Trichoderma reeseistrains QM9414 and Rut-C30 were constructed in which the genes for the two major hydrophobin proteins, hydrophobins I (HFBI) and II (HFBII), were deleted or amplified by molecular biological techniques. Growth parameters and foam production of the transformant strains were compar...

journal_title：Applied microbiology and biotechnology

authors： Bailey MJ,Askolin S,Hörhammer N,Tenkanen M,Linder M,Penttilä M,Nakari-Setälä T

更新日期：2002-05-01 00:00:00

abstract：:The tandem multimeric expression of various peptides has been explored by many researchers. However, expression levels have usually not been proportional to the degree of multimerization. To increase the expression level in Escherichia coli of tandem multimers of a cationic antimicrobial peptide, buforin II, fused to ...

journal_title：Applied microbiology and biotechnology

authors： Lee JH,Kim MS,Cho JH,Kim SC

更新日期：2002-05-01 00:00:00

abstract：:Diastereoselective reduction of diketones with Lactobacillus kefir DSM 20587 was examined. The reduction of both oxo-functions proceeded highly diastereoselectively. (2 R,5 R)-Hexanediol 3 was produced starting from (2,5)-hexanedione 1 in quantitative yields with enantiomeric excess >99% and diastereomeric excess >99%...

journal_title：Applied microbiology and biotechnology

authors： Haberland J,Kriegesmann A,Wolfram E,Hummel W,Liese A

更新日期：2002-04-01 00:00:00

abstract：:A family I cellulose-binding domain (CBD) and a serine- and threonine-rich linker peptide were cloned from the fungi Aspergillus japonicus and Aspergillus aculeatus. A glutathione S-transferase (GST) fusion protein comprising GST and a peptide linker with the CBD fused to its C-terminus, was expressed in Escherichia c...

journal_title：Applied microbiology and biotechnology

authors： Quentin M,Ebbelaar M,Derksen J,Mariani C,van Der Valk H

更新日期：2002-04-01 00:00:00

abstract：:The exclusive use of isopropyl beta-D-thiogalactopyranoside to activate the T7 promoter for protein production has limited the general use of the expression system. We have sought an alternative by constructing a recombinant Escherichia coli strain, BL21 (G2), to carry a chromosomal copy of T7 gene 1 fused to the lamb...

journal_title：Applied microbiology and biotechnology

authors： Chao YP,Law W,Chen PT,Hung WB

更新日期：2002-03-01 00:00:00

abstract：:The fungal conversions of sulfur-containing heterocyclic compounds were investigated using the lignin-degrading basidiomycete Coriolus versicolor. The fungus metabolized a series of sulfur compounds--25 structurally related thiophene derivatives--via several different pathways. Under primary metabolic conditions, C. v...

journal_title：Applied microbiology and biotechnology

authors： Ichinose H,Nakamizo M,Wariishi H,Tanaka H

更新日期：2002-03-01 00:00:00

abstract：:A newly isolated strain of Cunninghamella echinulata grown on glucose produced significant quantities of biomass and cellular lipids in media with high C/N ratio. The oil yield from glucose consumed increased after nitrogen exhaustion in the growth medium, but gamma-linolenic acid (GLA) content in cellular oil systema...

journal_title：Applied microbiology and biotechnology

authors： Gema H,Kavadia A,Dimou D,Tsagou V,Komaitis M,Aggelis G

更新日期：2002-03-01 00:00:00

abstract：:The alkali-soluble glucan of the yeast cell wall contains beta-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of beta-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutant...

journal_title：Applied microbiology and biotechnology

authors： Ha CH,Lim KH,Kim YT,Lim ST,Kim CW,Chang HI

更新日期：2002-03-01 00:00:00

abstract：:Twenty-three genotypes of maitake (Grifola frondosa) from Asia, North America and Europe were compared for biological efficiency (BE), quality and crop cycle time. Significant differences among lines were found for BE and crop cycle time when mushrooms were produced on nutrient-supplemented (15% millet, 10% wheat bran...

journal_title：Applied microbiology and biotechnology

authors： Shen Q,Royse DJ

更新日期：2002-02-01 00:00:00

abstract：:Bacteria that grow on p-xylene, p-toluic acid, and terephthalic acid (TPA) were isolated from a wastewater bioreactor that is used to treat a waste stream that contains all three of these compounds. Although previously described aerobic bacteria degrade p-xylene by initially oxidizing a single methyl group to form p-t...

journal_title：Applied microbiology and biotechnology

authors： Bramucci MG,McCutchen CM,Singh M,Thomas SM,Larsen BS,Buckholz J,Nagarajan V

更新日期：2002-02-01 00:00:00

abstract：:Under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. The phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. The additi...

journal_title：Applied microbiology and biotechnology

authors： Hollender J,Dreyer U,Kornberger L,Kämpfer P,Dott W

更新日期：2002-01-01 00:00:00

abstract：:Using a reverse-transcription polymerase chain reaction (RT-PCR) technique, cytochrome P450 genes were cloned from the lignin-degrading basidiomycete, Coriolus versicolor. One possible P450 gene was identified, which consisted of 1,672 nucleotides and a poly(A) tail and encoded a deduced protein containing 449 amino a...

journal_title：Applied microbiology and biotechnology

authors： Ichinose H,Wariishi H,Tanaka H

更新日期：2002-01-01 00:00:00

abstract：:Synthetic logs were vacuum-soaked or regular-soaked to determine the effects of soaking on yield and mushroom size, log weight variability and water distribution within the log. Yields (g/log) from substrates vacuum-soaked were higher by 26.7%, 18.6% and 35.8% (mean = 27.2%) for crops I, II and III, respectively, when...

journal_title：Applied microbiology and biotechnology

authors： Royse DJ,Rhodes TW,Sanchez JE

更新日期：2002-01-01 00:00:00

abstract：:An immobilized-cell biofilm reactor was used for the continuous production of lactic acid by Lactobacillus casei subsp. rhamnosus (ATCC 11443). At Iowa State University, a unique plastic composite support (PCS) that stimulates biofilm formation has been developed. The optimized PCS blend for Lactobacillus contains 50%...

journal_title：Applied microbiology and biotechnology

authors： Cotton JC,Pometto AL 3rd,Gvozdenovic-Jeremic J

更新日期：2001-12-01 00:00:00

abstract：:A histidine oligopeptide (hexa-His) with the ability to chelate divalent heavy metal ions was displayed on the yeast cell surface for the purpose of enhanced adsorption of heavy metal ions. We genetically fused a hexa-His-encoding gene with the gene encoding the C-terminal half of alpha-agglutinin that includes a glyc...

journal_title：Applied microbiology and biotechnology

authors： Kuroda K,Shibasaki S,Ueda M,Tanaka A

更新日期：2001-12-01 00:00:00